Qingdao genetically modified somatic cell clone goats completed scientific identification

Qingdao genetically modified somatic cell clone goats completed scientific identification

On April 10th, Qingdao Science and Technology Bureau organized experts to pass the appraisal of the “Development of Laoshan Dairy Goat Mammary Gland Bioreactor” undertaken by Qingdao Senxi Industrial Co., Ltd., so that Qingdao genetically modified somatic cell clone goats have completed scientific identification. It lays an important foundation for the industrialization of pharmaceutical proteins. The use of mammary gland bioreactors to clone milk goats from genetically engineered somatic goats to extract pharmaceutical protein products has the characteristics of high yield, low investment, low cost, easy purification of products, high biological activity and safety. At present, only a few countries in the world have made significant progress in the clinical trials of pharmaceutical protein products in bioreactors or in the market. Qingdao Senhao Industrial Co., Ltd. has invested nearly 10 million yuan for five years since April 2000. Under the support of the Qingdao Municipal Science and Technology Bureau and the Chinese Academy of Sciences and the National 973 Program, the Laoshan Dairy Goat is an experimental object and Qingdao Laoshan has been built. North House's 6,000-square-meter genetically modified animal experiment base forms a mature system of simultaneous estrus, superovulation, micromanipulation, embryo transfer, and experimental feeding and management systems. The construction of human hepatitis B surface antigen, β-interferon and antithrombin III transgenic vector and establishment of Laoshan dairy goat fetal fibroblast cell line were completed, and these three genes were successfully transformed into Laoshan dairy goat fetal fibroblasts. In the cells, cell lines with the above three genes were obtained. Using cell nuclear transfer technology, transgenic somatic cell cloned goats were successfully obtained. Eighteen transgenic goat somatic milk goats had survived so far. The first transgenic somatic cell cloned from male goats was "presented". It was born on September 28, 2002. It has a strong, vigorous skeleton and carries the beta-interferon gene. It has bred with a large number of selected Daishan dairy goats and ewes. A total of 23 offspring lambs were obtained, of which 9 were positive transgenic dairy goats. The third genetically modified somatic goat cloned on March 20, 2003 was a male goat. Shunshun”, carrying the antithrombin III gene, is often married to the Laoshan dairy goat ewes with a strong body, and has obtained 19 offspring lambs, and 9 of them are positive transgenic goats. They have created amazing achievements for the transgene somatic cell cloned goats.

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