VIE fish fluorescent labeling operation and use method

The VIE fish fluorescent label mainly uses a fluorescent compound having affinity with calcium to act on the labeling object, and deposits it on the otolith to form a fluorescent label recognizable under a fluorescence microscope. Since Douglas et al. discovered that tetracycline hydrochloride (TC) can be deposited on fish bones and scales, systematic research on chemical labeling of fish otoliths has begun. Chemical markers can be carried out in any life history of fish including fertilized eggs, and otoliths. The logo is permanently stored and is often used for large-scale marking.

The commonly used materials that have been screened so far have been oxytetracycline (OTC), tetracycline hydrochloride, alizarin complex (ALC), alizarin S (ARS), calcein, etc. [19], tetracyclines are the most used time. Long labeling substances, but the labeling effect is slightly inferior to ALC and ARS. Calcein can mark many fish well, but its safe concentration is small and the action time is difficult to grasp.

The VIE Fish Fluorescent Labeling Flow Marker consists of two parts of silicone resin and is mixed before use. The VIE marker line is a liquid prior to injection and quickly becomes a solid substance that adapts to the organism after injection into the body. These marking lines are placed underneath the transparent or translucent tissue to remain visible from the outside. On many amphibians, the VIE marking line can even be observed through black skin. VIE marking lines are widely used in more aquatic animals, fin whales, crustaceans, reptiles and amphibians.

VIE fish fluorescent labeling pathways have injection, soaking or feeding of markers containing feeds. Studies have shown that the labeling effect of alizarin feeding or injection is not satisfactory, and the soaking labeling effect is obvious, while the calcein is better than the labeling by injection. soak. Which markers and marking methods are generally used are generally based on the size of the labeled fish, the stage of life history, the number of markers, and the budget of the funds.

Otolith thermal labeling is a lifelong identifiable marker by artificially regulating the water temperature for a short period of time to alter the characteristics of one or more growth rings on the otolith. The embryonic and larva otoliths can be effectively and extensively labeled by artificially regulating the temperature fluctuations, and the method is simple and inexpensive. This marking technology has been widely used worldwide.

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