A new generation of SPR bio-molecular interaction analyzer innovation technology advantages of the inventory

Nicoya O pen SPR individual molecules interact on-line analyzer hot

- a new generation of LSPR technology to bring you non-general technology experience

The basis of life activities is the interaction between biomolecules and between biomolecules and other molecules. Study biomolecular interactions, such as proteins and proteins, proteins and nucleic acids, proteins and lipids, nucleic acids and peptides, and understand life phenomena at the molecular level, including cell functions, various physiological activities, pathologies, and mechanisms of action of drugs. , immune response and other mechanisms to clarify the mechanism of life activities, discover the nature of life, develop new drugs. This has very important theoretical and practical significance for human understanding and transformation of nature, understanding of human beings, discovery of pathogenic mechanisms and treatment pathways. DNA, protein, and lipids are the three main substances that make up the body. They store and transmit information, perform various functions in cells or in the body, and store and provide energy for the body to ensure the normal functioning of the body. The interaction between these molecules is helpful for understanding, controlling, treating genetic diseases and in vivo dysfunction, body function decay, tumorigenesis, antiviral immune response, etc. Studying the interaction between DNA and drug molecules is to understand the role of drugs. Mechanism, finding and discovering new drugs laid a solid foundation.

Leading the frontier of technology and complying with the requirements of the times, Nicoyalife has innovatively developed a new generation of SPR technology with nano gold particles as the detection unit, namely LSPR local plasmon resonance technology innovation. Different from the traditional SPR technology to detect the deflection of the SPR angle caused by the change of refractive index, LSPR detects the displacement of the light absorption peak caused by the change of the thickness of the molecular layer on the surface of the nano gold particles. Since the change of the wavelength of light is affected by the environment, the volume is The temperature, buffer refractive index and other changes are not sensitive to interference. Therefore, the detection results of this series of products ensure the accuracy and sensitivity similar to the traditional SPR technology, and the simpler optical path system and operation process are greatly reduced. The experimental threshold of SPR technology, the renewable chip also reduces the cost of the instrument to the later use. This series of advantages makes SPR technology promising as a technical platform support for life science research experiments.

• Nicoya's innovative technology - " LSPR " detection principle

• Simple experiment and chip regeneration process:

The SPR molecular interaction analyzer is fully controlled in 0.5 hours.

Figure 1. Molecular interaction kinetics detection process

• Accuracy of experimental results comparable to traditional SPR technology:

The same ligands M1 and M2 were tested by OpenSPR, IBIS and Biacore respectively. The analysis results show that the three instruments have comparable similar signal-to-noise ratios:

Figure 2. OpenSPR pair (M1, M2) coupling curve and kinetic analysis

Figure 3. IBIS pair (M1, M2) coupling curve and kinetic analysis

Figure 4. Biacore pair (M1, M2) coupling curve and kinetic analysis

【Result analysis】

OpenSPR, IBIS, M1 of Biacore instruments detected three K _D were 5.7nm, 5.2nm, 8.9nm, M2 is K _D respectively 0.54nm, 0.12nm, 0.16nm, an average K _D value M1 is 6.61nm +/- 2.0 Nm, M2 is 0.27+/0.23 nm. Comparative study of global standard molecular affinity biosensor that analyzes K _D value of the interaction of the antibody protein to the same by the client 150, the final result of the K _D 0.62nm +/- 0.98nm, so these three instruments The results are consistent in error. This error is predictable in the experiment due to differences in instrumentation, conditions, and user. It also proves that openSPR is more reliable than other systems.

• Core competitive advantage:

Affordable : the next generation portable molecular interaction analyzer that every lab can afford

Low cost of use: chip regenerable, unlimited reuse

Meet different experimental needs: 6 kinds of chips - blank nano gold, COOH, NH ₂ , NTA, Streptavidin, Biotin, for the interaction of different ligands and analytes

Easy to operate: fully controlled in 0.5 hours

Strong anti-interference ability: the detection is not affected by the external environment such as temperature and buffer refractive index.

Powerful: It can be used to analyze the interaction of large and small molecules such as proteins, nucleic acids, lipids and peptides.

Wide range of applications: biochemistry, drug development, cancer research, immunology research, antibody engineering, etc.

The company's contact information is as follows, sincerely look forward to working with you!

Purui Maddy (Beijing) Laboratory Technology Co., Ltd. Website:

Address: 401 International Business Incubation Center, No. 9 Kexing Road, Fengtai District, Beijing

telephone / fax