The perfect solution for clinical sample collection and stabilization

The perfect solution for clinical sample collection and stabilization

The necessity and solution of nucleic acid stabilization


Nucleic acid extraction is the most critical part of clinical PCR testing. The success or failure of nucleic acid extraction is related to the correctness of downstream PCR test results, and it is also the most problematic part of clinical PCR test operations. High quality nucleic acid quality, standardized nucleic acid purification processes and traceability of the entire process are the top priorities of the entire molecular diagnostic process. As a leading brand in the field of nucleic acid purification, QIAGEN's products and performance are highly compatible with all the requirements for sample preparation in the field of molecular diagnostics.

After the biological sample is collected, the RNA content will be changed by many factors, such as degradation by RNase, or by external factors such as temperature and buffer. QIAGEN provides a series of products to rapidly stabilize RNA. Allprotect Tissue Reagent is able to stabilize DNA/RNA/protein in animal tissues at the same time. PAXgene Tissue Container also fixes tissue and has the same effect as formalin. The use of these stabilizers ensures true gene expression profile data and experimental reproducibility (Figures 2, 3).

  • Fast and stable RNA
  • Safe handling of samples at room temperature - no liquid nitrogen or dry ice required
  • Convenient sample transport - at room temperature
  • Suitable for long-term preservation of samples

Figure 2 Rat kidney tissue was stabilized by RNeasy Protect Mini Kit and allowed to be purified to complete RNA after 60 minutes at room temperature. RNA was degraded after a period of tissue placement using conventional methods.

Figure 3 RNAprotect® Saliva Reagent stabilizes the level of β-actin mRNA in saliva, which is quantified by real-time RT-PCR.

Figure 4 Allprotect stable mechanism: Each Allprotector molecule has multiple contacts that allow the Allprotector molecule to coat and protect DNA, RNA and proteins. When the tissue is lysed, the Allprotector molecule is diluted and decomposed to release DNA, RNA and protein.

Sample Stabilizer Selection Guide

RNA stabilization reagent

sample

RNA Stabilization Reagent Name

Item number

organization

RNAlater RNA Stabilization Reagent

76104

RNAlater TissueProtect Tubes

76154

PAXgene Tissue Container

765112

Allprotect Tissue Reagent

76405

cell

RNAprotect Cell Reagent

76526

bacterial

RNAprotect Bacteria Reagent

76506

saliva

RNAprotect Saliva Reagent†

blood

PAXgene Blood RNA Tubes

762165

marrow

PAXgene Bone Marrow RNA Tubes

764114


RNA Stabilization and Purification Kit


sample

RNA Stabilization and Purification Reagent Name

Item number

organization

RNeasy Protect Mini Kit

74124

cell

RNeasy Protect Cell Mini Kit

74624

bacterial

RNeasy Protect Bacteria Mini Kit

74524

saliva

RNeasy Protect Saliva Mini Kit

74324

blood

PAXgene Blood RNA Kit*

762174

marrow

PAXgene Bone Marrow RNA Kit*

764133


* Dedicated to PAXgene Tube stable blood, † included in 74324.

The above information comes from QIAGEN official website!

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