Release date: 2017-09-01 The Associate Professor of the University of Science and Technology of China has made significant progress in NMR research on medium-affinity chemical tool molecules and target protein interaction patterns. The relevant research results were published online in "Applied Chemistry of Germany" on August 29th. The co-first authors of the paper are Gao Jia of the Chinese University of Science and Technology and Liang E, a doctoral student of Tsinghua University. The protein-ligand interaction pattern is at the heart of rational drug discovery. The study of crystal structure of complexes is limited by the water solubility of small molecules, while the lead compounds of moderate affinity are usually in the nuclear-scale exchange scale, resulting in a serious widening or even inability to detect the nuclear magnetic resonance signal lines at the interface. The pattern of action with target proteins poses a serious challenge. The researchers used a very low protein-ligand molar ratio to alleviate the spectral broadening effect caused by chemical exchange, and the 19F chemical exchange saturation transfer (CEST) was used to determine the low-abundance bound lanthanide-labeled BRM bromo. 19Fèµ contact displacement (PCS) of single F and double F small molecule inhibitors of the domain. The researchers named this new method 19F PCS-CEST, which provides important structural constraints on protein-ligands to select the best binding mode for molecular docking and complex crystals with another highly water-soluble analog. The structure is consistent. This method solves the problem of the NMR characterization of the combination mode of the lead compound in the medium affinity range with the target, and expands the application of NMR in the structure-directed evolution of the lead compound to the lead compound. The work was funded by the Chinese Academy of Sciences' Strategic Pilot Science and Technology Project, National Key Research and Development Project, 973 and the National Natural Science Foundation. Source: University of Science and Technology of China Tetanus Toxoid Vaccine,Toxoid Vaccine,Hep B Immune Globulin,Immunoglobulin Injections FOSHAN PHARMA CO., LTD. , https://www.full-pharma.com
Study on the realization of medium-affinity ligand and target protein mode of action by nuclear magnetic resonance