Precautions for packing packing

Carefully look at the instructions for use of the packing before packing. Each packing has its own characteristics and a special packing method. For different empty columns, the method may be different.

1. Before packing the column, carefully read the instructions for the packing. Each packing has its own characteristics and a special packing method. For different empty columns, the method may be different.

2 , the filler should be added with water to stir into a uniform suspension, the concentration of the suspension varies depending on the filler, generally 70-85% , quickly poured into the empty column, or suction or pressure, flow rate and specific steps to see the instructions.

3 , this is very important: loading the column, including in the future use, absolutely does not allow bubbles to enter the column (bubble free) , the column head should also exclude bubbles beforehand.

4 , choose the right pump, first of all, the flow rate meets the requirements, the pulse is as small as possible. The preferred plunger pump.

5. All columns should be as uniform as possible, especially for gel chromatography. After the column is completed, it can be checked by the method of column efficiency measurement.

Check the filling effect. The theoretical plate height of a loaded column should be 2-4 of the average particle size of the filler.   Times, the smaller the better in this range

6. The liquid to be applied to the column should be filtered, at least 0.45 micrometre .

7 , as for sequencing, I think it is less than 1mg   It should be enough.

8. Regarding purification, the first thing to consider is your goal: purity, quantity, speed, yield, etc., and then determine the purification strategy according to the specific conditions of the sample, especially the nature of the target protein. Usually three steps: capture , purification , polishing. The first step is to “grab” the target protein from a large or complex sample and separate it from the main impurities; then remove most of the impurities with high-selectivity chromatography; finally finely purify to provide purity to the set target. In actual work, don't rely on three steps, maybe one step or four steps or more, and analyze the specific situation. The so-called "three steps" is just an idea.

Organic Buckwheat

Buckwheat is nutritious and full of treasures. Nutrition experts identified: buckwheat contains 13.1% protein, 17.7% vitamins, 18 kinds of amino acids are complete, and the content of lysine and tryptophan is unmatched by other food crops. Calcium, phosphorus, magnesium, iron, zinc, selenium and other trace elements are complete, especially suitable for hypertension, dyslipidemia, coronary heart disease, diabetes, obesity, arteriosclerosis, loss of appetite, gastrointestinal stagnation, chronic diarrhea and night sweats The first choice for patients. Buckwheat also has antibacterial, anti-inflammatory, cough, asthma, and phlegm-relieving effects, so buckwheat is also known as "anti-inflammatory food".