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1, using a one-time exchange module
The switching microfluidic module used by On-chip is an integral design containing a sheath liquid tank and a waste liquid tank, and is made of acrylic resin. Since On-chip is available in both analytical and sorting models, it is also possible to select the appropriate module and whether it has been aseptically processed according to the experimental needs. 
The sorting structure is not in the micro-module, and the air pump is installed in the device and the high-speed electromagnetic pulse is used to form the air pressure to control the flow of the sample in the module, thereby achieving cost reduction.
On-chip Sort can be upgraded to up to 3 laser 6-color 8 parameters. Selectable lasers include 405 nm, 488 nm, 561 nm, 637 nm, and 785 nm. The standard configuration is a combination of 488 nm, 561 nm, and 637 nm. When the switch module is equipped with a CCD camera that prevents positional shift and tilt, the position of the flow path can be confirmed. When examining side astigmatism, a 45 degree tilt causes the side astigmatism to achieve total reflection down, developing a unique optical loop (SLER optical loop). Use the CCD camera to confirm the flow path and laser position, and automatically adjust the optical path for easy use.
The fetal brain hippocampal neurons of mice were sorted by On-chip Sort. The cells sorted by the traditional sorting method (left) are basically dead after 7 days of culture, and the cells isolated by our equipment (right) are not damaged, and the ability of the nerve antenna to expand and contract can be seen. 
On-chip aseptic, maintenance-free, flow-through cell sorter principle and new application
The traditional high-pressure charge sorting of the flow will cause some damage to the cells, which causes great inconvenience to the original performance and characteristics of the research cells. In response to this drawback, Japan On-chip Biotech Co., Ltd. launched a pollution-free, maintenance-free flow cytometer (On-Chip) that does not damage cells and ensures clinical application in 2012. This small instrument uses a microfluidic chip module that uses air pressure control to separate cells from the sample stream in the module, with up to 3 lasers and 6 fluorescence. The cells are sorted at a low pressure to achieve a non-invasive separation. The disposable exchange type module ensures that there is no pollution between the samples, and there is no need to clean the flow path, which is simple and convenient.
Innovation
2, the principle of sorting
As shown in the figure below, the sample solution is given a normal air pressure, and flows at a speed of about 500 mm/sec. After the sheath liquid is combined, it passes through a laser irradiation area to determine whether it is a target cell. The pulsed air pressure is applied at the moment of passing through the cross-shaped sorting circuit on the lower side, so that the target cells enter the recovery circuit on the Pull side at a high speed. The liquid pressure pushed by the sample is about 2 kPa based on atmospheric pressure, which is 1/100 of the pressure of the conventional flow cytometer; the pressure at the sorting is lower than that of the normal flow cytometer to push the liquid, about 50 kPa or less. Therefore, On-chip Sort does not form the phenomenon of high pressure, dripping, and negative charge during the conventional flow sorting, thereby achieving cell-free damage sorting.
3. Innovative light path for cell analysis
4, no damage sorting application examples
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