Dr. Yang Liuqing, a Chinese scholar at the University of Texas Anderson Cancer Center, is mainly engaged in basic research in tumor metastasis and signaling. Recently, Dr. Yang Liuqing analyzed the expression of long-chain non-coding RNA (lncRNA) in breast cancer tissues using the lncRNA chip of Arraystar, USA, and found that some lncRNA can directly interact with lipids (phosphatidylinositol triphosphate PIP3, lecithin PC, etc. ) Interactions occur to regulate breast cancer. The authors also delved into the molecular mechanism of the interaction between lncRNA LINK-A and phosphatidylinositol triphosphate (PIP3), providing a potential target for the treatment of breast cancer. The research results were published in 2017 in the Nature issue of Nature Cell Biology (IF=18.699). Research Background Research ideas Technical route Result display Figure 1. Identification of lncRNA binding to lipid molecules Figure 2 Lipid-lncRNA pull down experiments enriched 9 abundance lncRNA Figure 3 LOF experiment to verify the effect of LINK-A on tumor growth Significance about the author Original source Food Grade Sorbates,Potassium Sorbate,Food Grade Potassium Sorbate,Sorbistat Potassium Wuxi Yangshan Biochemical Co.,Ltd. , https://www.yangshanchem.com
(Chip experiments are provided by Arraystar) 
lncRNA plays an important role in the regulation of signaling pathways, and in most cases lncRNA needs to function by binding to proteins. However, recent research evidence suggests that a small number of lncRNAs may be involved in the interaction of lipids and interactions. For example, some lncRNAs bound to the Saccharomyces cerevisiae cell membrane can regulate the permeability of cell membranes; however, it has not been identified that it can directly bind to lncRNA. Phospholipid molecule. The purpose of the author in this article is to identify lncRNAs that bind to lipids, and elaborate on the molecular mechanisms by which they bind, and the possible clinical implications.
To find lncRNAs that specifically bind to lipids, the authors extracted RNA from normal breast and breast cancer tissues and lipid fractions isolated from these tissues, and analyzed them using the human lncRNA chip from Arraystar, USA. The expression level of part of lncRNA. The results of the microarray analysis showed that 9 of the 550 lncRNAs with significant differences were lipid-related and were verified by qPCR. Among them, LINC01139 has the highest concentration in the lipid component. Therefore, the authors have studied the interaction between lncRNA LINC01139 (LINK-A) and lipid molecules.
Through pull down-qPCR experiments, the authors found that the abundance of LINK-A in PC and PIP3 pull down RNA is relatively high, and experiments have shown that the affinity of LINK-A and PIP3 is higher than that of PIP3 and protein. At the same time, the authors also found that LINK-A promotes the binding of AKT and PIP3, thereby activating AKT kinase, and the activation of AKT-PIP3 is highly correlated with tumor growth and metastasis, and further discusses the LINK-A-PIP3-AKT binding molecule. mechanism.
Finally, the authors verified the biological function of LINK-A by LOF experiments, and found that the decreased expression level of LINK-A would cause the tumor glucose transport system to be blocked and the tumor volume to be smaller. This suggests that LINK-A is an indispensable molecule for the maintenance of tumor cell proliferation and anti-apoptosis, and also provides a potential target for the treatment of breast cancer. 
The left panel is the experimental flow of high-throughput screening and lipid-binding lncRNA, and the right panel shows the lncRNA that binds to lipids and is abnormally expressed. 
In this study, a large number of lncRNAs abnormally expressed in breast cancer tissues were screened by the human lncRNA chip of Arraystar, USA. Studies have shown that lncRNA functions by binding with lipid molecules and deeply analyzes its mechanism of action. In addition, the authors used LNA (locked nucleic acid) technology to knock down the expression of LINK-A, found that the glucose transport system of tumor cells was blocked, and the proliferation of tumors was inhibited, which provided a potential target for the treatment of breast cancer.
Dr. Yang Liuqing, Assistant Professor, University of Texas Anderson Cancer Center. The main research areas: tumor metastasis and signal transduction, published more than 20 SCI articles in recent years.
The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors. Nature Cell Biology. 2017.
Nature Subsidiary: Arraystar lncRNA Chip for Breast Cancer Research