MAP1B monoclonal antibody product manual (Chinese version)

MAP1B monoclonal antibody product manual (Chinese version)

MAP1B monoclonal antibody product manual (Chinese version)

The main purpose

The MAP1B monoclonal antibody is a murine monoclonal IgG antibody protein designed for immunohistochemistry, immunoprecipitation and western blotting experiments. Commonly used in immunofluorescence, cell flow, Western Blot, immunohistochemistry, immunoprecipitation and other experiments. The product is ready to use, stable performance and guaranteed immune activity.

technical background

Microtubule associated protein (MAP) is a group of related proteins that make up microtubules, including MAP1, MAP2, MAP3, MAP4, MAP5, and tau protein. Among them, MAP1B, also known as MAP5, is the main tubulin in brain development, especially in the brain of newborns. Mouse anti-MAP1B (NP_005900.2; GeneID 4131) monoclonal IgG antibody (3G5), which is not immunoreactive with tubulin and other microtubule-associated proteins. It can be cross-reactive with mice, rats, humans, and the like.

product content

Antibody solution (Reagent A) (200 μg/ml) 100 μl

Product manual 1 copy

storage method

Stored in a -20 ° C refrigerator, effective for June

Experimental tips

  • Immunoprecipitation: 2 to 5 μg / 1 mg of lysed protein or ml
  • Western Blot: 1:500; 270Kd
  • Cell flow: 1 μg/10 6 cells
  • Immunofluorescence: 10 μg/ml
  • Immunohistochemistry: 1:1000
  • Can be used for frozen sections, etc. after formalin or paraformaldehyde fixation

Precautions

  • This product is 100 micrograms of protein specification
  • Wear gloves when handling
  • If turbidity occurs, use a positive control that can be used for enzyme activity detection after centrifugation.
  • The user adjusts the reagent dosage ratio according to the actual situation.
  • Strictly avoid repeated freezing and thawing; temporarily not used, kept in the refrigerator at -20 °C
  • The company provides a series of antibody reagent products

Quality Standard

  • This product has been certified to be stable.
  • This product has been certified to ensure response

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