How to shorten the slow seedling stage of rice

After transplanting the seedlings, due to root injury, the water absorption and fat absorption capacity decreased, the aboveground part stopped growing, the leaf color turned yellow, and the roots began to grow for 3-4 days after transplanting, and the root absorption capacity gradually recovered, and the leaves turned from yellow to green. The new leaves begin to grow, and this time is called the slow seedling period. The shorter the seedling period, the better. The length of the slow seedling period is related to the seedling's own situation and the method of moving the seedlings. It also has a great relationship with the management of the slow seedling period. In order to shorten the slow seedling period, the technical measures after transplanting are as follows:

1, check seedlings

After the insertion, the whole field was inspected and found to be dribbled and dumped in time.

2, irrigation

Immediately after the insertion, the deep water is poured, and the water depth is half of the height of the seedling. The purpose is to reduce the transpiration of the leaves and prevent the water imbalance in the seedlings. After 2-3 days, keep the shallow layer of water 2-3cm to increase the ground temperature and promote the early growth of new roots and new leaves. In the paddy field irrigated by well water, due to the low water temperature, irrigation can be carried out by extending the waterway, changing the water inlet, and supplementing the water to increase the water temperature.

3, top dressing

A week or so after transplanting, a small amount of chemical fertilizer can be applied, and 3-4 kg of urea can be chased to promote the growth of new leaves. Maintain a water layer of 2-3 cm when topdressing. This period of application of the base fertilizer before transplanting may not be applied.

4, weeding

5-7 days after transplanting, use 50% of the herbicide emulsifiable concentrate 500g per mu, or mix it with 96% of the big emulsifiable concentrate 150g, and apply it before the second leaf of Valerian. In addition, weeding can also be combined with cultivating.

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Nucleic Acid (DNA/RNA) Extraction Kit

1. Introduction

The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:

1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.

2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.

3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.

4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.

3. Shelf life

Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.

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