How to choose the appropriate VSV-G tag antibody?

The vesicular stomatitis virus (VSV) belongs to the enveloped RNA virus and is released from the plasma membrane of the host cell by budding. The fusion coat G glycoprotein (VSV-G) derived from vesicular stomatitis virus has been used as an important means of gene transfer in retrovirus and lentiviral vectors. The VSV-G tag (sequence: YTDIEMNRLGK) is derived from a portion of the VSV-G protein and is often used to fuse at the N-terminus or C-terminus of the protein of interest and is conveniently visualized by immunohistochemistry.

Figure 1: Various labels

How to choose the appropriate VSV-G tag antibody?

When choosing VSV-G antibodies, we mainly rely on our own needs, especially the needs of experimental applications. In addition, the more types of applications after an antibody is detected, the greater the choice in use; while the monoclonal antibodies from mouse sources generally have better specificity, stability, and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100μl VSV-G antibody (if the dilution ratio of WB is 1:5000, the final use solution is equivalent to 500ml), 1ml of VSV-G antibody with lower potency (if the dilution ratio of WB detection) It is 1:200, and the final use liquid is equivalent to 200ml).

Product name and description	Item number	product manual
Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2)	A02180	Details

Why recommend the Abbkine brand VSV-G monoclonal antibody (clone number 14D2)?

At present, the VSV-G antibody available on the market has domestic packaging or original production. The domestically packaged VSV-G antibody has insufficient quality stability, and after the packaging, its potency is also lost. Some VSV-G antibodies originating in domestic laboratories have better detection activity, but there are gaps in application types, and it is difficult to control the stability between batches, while only a small amount of origin is produced in China. Antibodies are also polyclonal antibodies. As for the imported imported VSV-G antibody, there are many imported brands, such as Abcam, Bio-Rad, Novus, Thermo, etc., which are related to VSV-G antibody, but they are far less expensive than Abbkine products.

Therefore, we recommend Abbkine's VSV-G-tag antibody, Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2). Like Abcam, the immunogen of Abbkine's VSV-G antibody is KLH-conjugated VSV-G. The tagged YTDIEMNRLGK polypeptide sequence, which has been continuously screened and optimized, finally yields the best cell line (14D2), which recognizes the VSV-G protein and the mCherry fusion protein overexpressed in cells, which is less than half the price of Abcam. As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing. The antibody can be used in Western Bloting, immunofluorescence IF, and immunoprecipitation IP experiments.

Figure 2: Antibody Verification Results (Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2))

In addition to a wide range of applications, Abbkine also offers 1ml large packaging and larger custom-made VSV-G-labeled antibodies, making it ideal for routine testing of VSV-G immunoassays. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact Abbekine China distributor Amy Jie Technology Co., Ltd. directly.

The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its general agent in China and will provide customers with the most cost-effective routine immunological reagents, such as A variety of tag antibodies, internal reference antibodies, conventional primary antibodies and a full range of secondary antibodies, antibody detection substrates and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.