How do clinicians send SCIs with more than 10 points?

The doctor is a hard-working profession that wants to see a doctor again, and to do surgery and stay up late to read the literature. Dr.S is deeply sympathetic. In order to help the doctors who have limited energy to focus on the clinic to do better research, Dr.S will continue to share a series of high-scoring SCI articles published in recent years. Analyze the ideas and routines, and see how the excellent clinical research is done. Today, let's start with the lung cancer with the highest incidence in China.

This article shared today is a Journal of the National Cancer Institute published in 2017. The title is Association of Uba6-Specific-E2 (USE1) With Lung Tumorigenesis. It’s so simple and rude, it’s really daring because Without a complete story and a lot of data, it will be the embarrassment of the reviewer to catch the little sister-in-law. Therefore, it is recommended that you read the official in the writing of your own article, as far as possible in accordance with the "A gene through the B signal pathway in the D disease in the D function" mode, more specific, will not be reviewed by the reviewer is too broad.

First of all, the author does not know where to find a gene USE1 (Dr.S Tip: Generally, these new functional genes are sampled by high-throughput screening or excavated through the database), and the protein expression of this gene was found. The level is significantly elevated in lung cancer tissues relative to normal tissues. And it has several mutant proteins that are also significantly elevated in cancerous tissues. However, the mRNA level did not change much, indicating that there may be post-transcriptional regulation involved.

Since this gene is clinically relevant in lung cancer, does this gene have any function?

After it interferes, the ability of cells to proliferate and metastasize in vitro is reduced, and the ability to form tumors in vivo is also reduced.

After over-expression, the results were completely reversed, indicating that the gene is a proto-oncogene at both in vitro and in vivo levels. (Dr.S Tips: In fact, it is still difficult to get positive results in both interference and overexpression. It may take several repeated experiments to explore the best conditions, but it is recommended to verify the gene function in order to publish high scores.)

If you don't pursue it, you can make an article with this step, but you can make a 10-point article with your teeth. Is it a heartbeat, then what do we do below? Find an interaction protein!

What we found through mass spectrometry - CDC20 and CDH1, the star gene is woody, and CDC20 and CDH1 can also form APC/C complex to degrade the protein with D-box. Coincidentally, USE1 has D-box. Domain.

An interesting story just happened. If the USE1 mutation in the cell changes and the structure of the D-box changes, it is not easily degraded by APC/C. The accumulated USE1 can cause abnormal cell proliferation, invasion and metastasis, and promote lung cancer.

The fly in the ointment is that the author did not explain the mechanism of abnormal expression of wild-type USE1 causing abnormal proliferation, invasion and metastasis of lung cancer cells. It may be necessary to send another article.

Interpretation of Dr.S:

1. The biggest innovation in this paper is that the authors studied a coding gene, USE1, which has never been studied in lung cancer, and first reported its clinical relevance and function in lung cancer.

2. There is a new functional gene in the hand of a few articles, you have the final say, want to send high-scoring articles, the number of clinical samples and downstream mechanisms are indispensable.

3. There are also tricks to doing the mechanism. Screening downstream pathways or interacting proteins with high-throughput methods (chip or protein profiles) is often a multiplier.

4. The advantage of coding gene research is that it is often more powerful than non-coding genes. It is easier to make a positive result in a functional experiment. I don't tell him about it.

According to the experience of the Jikai gene, the probability of high-throughput screening of new functional genes from the sample by chip, sequencing, and mass spectrometry is about 10%. It is very difficult to screen and verify a new functional gene. After three years of research and development, Jikai Gene has invested hundreds of millions of dollars and has completed the functional screening of nearly 6000 tumor-related genes. From this, we have found a number of clear tumor function marker genes, waiting for you to hunt for treasure~~~

Long press and pay attention

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