High-resolution magnetic mass spectrometer application report and case in the environmental field (below)

High-resolution magnetic mass spectrometer application report and case in the environmental field (below)

Application Case 3: Polycyclic Aromatic Hydrocarbons

Instrument method:

Injector: Split/splitless, 1 min, 320°C, 1.5 μL
Injection volume with Merlin seal
Carrier gas: He, 0.6 mL/min, const. flow
Column: TRACE TM -50MS, 10 mx 0.1 mm x 0.1 μm
Oven Temp. Program: 140 °C , 1 min
10 °C /min to 240°C
5 °C /min to 270°C
30 °C /min to 280°C
4 °C /min to 290°C
30 °C /min to 315 °C
3 °C /min to 330°C
Transfer line 300°C
Ion source 280°C
Ionization: EI, 45 eV pos.
Scan Mode: Multiple ion detection mode (MID)
Resolution: 8,000, 10% valley definition
Cycle Time: 0.8 s/scan

references
[Application Note: 30126] Fast GC/HRMS Quantification of 16 EC Priority PAH ComponentsKarl Speer, Technical University Dresden, Institute of Food Chemistry, Dresden, Germany, Katja Ziegenhals, Wolfgang Jira, Federal Research Centre for Nutrition and Food (BfEL), Kulmbach , Germany,
Hans-Joachim Huebschmann, Thermo Fisher Scientific, Bremen, Germany
Contents of polycyclic aromatic hydrocarbons (PAH) and phenolic substances in Frankfurter-type sausages depending on smoking conditions using glow smoke
Margarete Pöhlmann , Alexander Hitzel , Fredi Schwägele , Karl Speer , Wolfgang Jira
Polycyclic aromatic hydrocarbons (PAH) and phenolic substances in meat products smoked with different types of wood and smoking spices Alexander Hitzel , Margarete Pöhlmann , Fredi Schwägele , Karl Speer , Wolfgang Jira

Application Case 4: Short-chain chlorinated paraffin

SCCP C 10 H 17 Cl 5 – “CP3” Reference Material Chromatogram

Sample Preparation

Sample preparation and standards are provided by Environment Canada, Burlington ON, Canada.
1. Reference material SCCP C 10 H 17 Cl 5 – “CP3”
2. Mixture SCCPs C10-13 55.5% Cl cyclohexane
3. Matrix extraction sample containing C13 internal standard

Gas Chromatography

GC Column: Trace Gold TR-5MS
30m x 0.25mm, 0.1 μm film
GC Liner: Single taper splitless
Injector temp: 220 °C
Splitless time: 3 minutes
Transfer line: 220 ° C
Column Flow: 1 mLmin -1 , constant flow

Mass Spectrometry

Electron capture negative chemical ionisation
Was used to analyse SCCPs.
EC NCI Buffer Gas: Isobutane and ammonia
Source Pressure: 6x10-4 mBar
Electron engery: 90 eV
Emission current: 0.3 mA
Ion source temp: 80-95 °C
Scan Multiple Ion Detection (MID)

references
Analysis of Short Chain Chlorinated Paraffins (SCCPs) using High Resolution Mass Spectrometry and Negative Chemical Ionization (NCI GC-HRMS)
Frank Theobald1, Heinz Mehlmann2, Dirk Krumwiede2 & Kyle D'Silva2 1. Environmental Consulting, Cologne, Germany 2. POPs Center of Excellence, Thermo Fisher Scientific, Bremen, Germany

Application Case 5: Hormonal steroids and pesticides

● EPA Method 1698: Steroids and Hormones in Water, Soil, Sediment, and Biosolids by HRGC/HRMS
Analysis of steroids and hormones in water, soil, sediments and organisms by high-resolution gas chromatography coupled with high-resolution magnetic mass spectrometry ● EPA Method 1699: Pesticides in Water, Soil, Sediment, Biosolids, and Tissue by HRGC/HRMS
Analysis of pesticides in water, soil, sediment and organisms by high resolution gas chromatography combined with high resolution magnetic mass spectrometry

References ● EPA Method 1698: Steroids and Hormones in Water, Soil, Sediment, and Biosolids by HRGC/HRMS
● EPA Method 1699: Pesticides in Water, Soil, Sediment, Biosolids, and Tissue by HRGC/HRMS

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